Protrusions; the role of actin cytoskeleton

Abstract

Singed (human Fascin), being an parallel bundle actin cross linker, is required for cell motility in both Droso¬phila and mammalian systems, as Fascin 1–dependent actin bundles are required for the formation of filopodia and invadopodia. Actin polymerization is the process through which the protrusions are formed but the stabilization of the protrusions are, perhaps, determined by the stability of the parallel bundles. Performing different experiments in this project we have come to know about the potential role of different actin cross linker proteins in the cell migration during protrusion formation. Various data from this project also clarify the role of hyper expressed singed in the border cell migration. We have to some extent found the redundant protein of singed which is the vinculin protein as the double knock down result of both singed and vinculin gene affects the border cell migration almost two folds greater than that of their individual knock down effect.