Design and Synthesis of some Fluorescent Probes studies with Biomacromolecular Systems

Abstract

Fluorescent probes are powerful tools for biosensing and bioimaging because of their high sensitivity, specificity, high fluorescence intensity, excellent solubility, biocompatibility and simple preparation. Hence, development of fluorescent probes specifically for biological settings and clinical setting has attracted intense interest. They can provide direct visualization and dynamic information concerning the localization and quantity of biomolecules of interest. Till to date, different kind of fluorescent probes is commercially available and can be used in biological investigations. ‘Click reaction’ is one of our main strategy for the synthesis of fluorescent probes. Click reaction is Cu(I)-catalyzed cycloaddition of alkyne and azide (CuAAC) reaction and by the CuAAC reaction, 1,2,3-triazole moiety-containing compounds can be synthesized conveniently. This triazole moiety have enormous application including pharmaceuticals, and are available as drugs today. Bovine serum albumin (BSA) and human serum albumin (HSA) are the most abundant plasma proteins which play a key role in many biological systems. The molecular weight of these two serum albumins (SA) are almost similar (66 KDa and 66.5 KDa are the molecular weight of BSA and HSA respectively) and also shows 80% structure similarity with each other. Protein ligand binding study is very important in drug design and developing system. Information is obtained from this study, whether it can act as a drug or not at very preliminary stage of research. Photophysical studies of protein ligand interaction using different spectroscopic technique enhance our knowledge towards the way of binding. Our research work mainly categorized into two parts namely, the synthesis of some novel compounds using standard methods such as click reaction, and thereafter investigation of the interaction of the synthesized compounds with serum albumins (e.g. BSA/HSA) by different spectroscopic technique and also the biological activities of the compounds. Hence, an attempt is taken for the binding study of the newly synthesized compounds with biomolecules to assemble the information about the role of a novel compounds for development of biomedicines in the drug design or drug delivery process at very preliminary stage of the modern research